Journal: Journal of Pain Research

Article Title: Melatonin MT2 receptor agonist IIK-7 produces antinociception by modulation of ROS and suppression of spinal microglial activation in neuropathic pain rats

doi: 10.2147/JPR.S214671

Figure Lengend Snippet: Continuous infusion of IIK-7 attenuated PSNT-induced activation of HMGB-1, pERK1/2, iNOS, STAT3 and caspase-3 in vivo. Representative immunoblots are HMGB-1, P44/42 MAPK, iNOS, STAT 3 and casp-3 from the left dorsal quadrant portion of the lumbar spinal cord lysate of sham surgery or PSNT rats continuously infused with either vehicle or IIK-7 for 7 days using an osmotic pump. Abbreviations: IIK-7, N-Butanoyl 2-(9-methoxy-6H-iso-indolo[2,1-a]indol-11-yl)-ethan-amine; P44/42 MAPK, P44/42 mitogen-activated protein kinase; iNOS, Inducible nitric oxide synthase; STAT3, Signal transducer and activator of transcription 3; CASP-3, caspase3; HMGB-1, High mobility group box 1.

Article Snippet: Primary antibodies against HMGB-1 (Wuhan Fine Biotech, Wuhan, China, Cat # FNab03924), p44/42 MAPK (Erk1/2) (Cell Signaling Technology, Beverly, MA, USA, Cat # 9102), STAT3 (Milliopre, USA Cat# 06–596), iNOS (Thermo Fischer Scientific, MA, USA, Cat# PA3-030A), casp-3 (Imgenex, Novus Biologicals, Littleton, CO, USA, Cat # 31A1067), β-actin (Sigma-Aldrich, St Louis, MO, USA) were incubated at 4°C overnight followed by the wash with TBST.

Techniques: Activation Assay, In Vivo, Western Blot

Journal: Yonsei Medical Journal

Article Title: Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model

doi: 10.3349/ymj.2016.57.5.1095

Figure Lengend Snippet: Morphologic changes of rabbit iliac artery analysis according to immunohistochemistry. Tissue staining of induced atherosclerosis iliac artery plaques in four groups of rabbits. The inflammation content of the plaques is detected by immunohistochemical staining for RAGE, HMGB1, and TNF-α; the macrophage content of the plaques is detected by immunohistochemical staining for the anti-rabbit macrophage clone RAM11. Representative examples of immunohistochemical stain of RAGE (A-D), HMGB1 (E-H), TNF-α (I-L), and RAM11 (M-P) in the rabbit iliac (amplification ×100) (scale bars=100 µm). Lesions of macrophage were markedly less for saline group and oil group, which showed significant differences in the total percent area in comparison to the HMGB1 group and TNF-α group on RAM11 immunohistochemical staining. * p <0.05, compared with the Saline group, † p <0.05, compared with the Oil group. RAGE, receptor for advanced glycation end products; HMGB1, high-mobility group protein B1; TNF-α, tumor necrosis factor-α.

Article Snippet: Membranes were blocked by a 5% skim milk (Noble Bio, Hwaseong, Korea) dilution in Tris-Buffered Saline and Tween 20 (TBS-T) at room temperature for 1 hour, and were subsequently washed three times in TBS-T. Membranes were incubated in TBS-T with primary antibodies against HMGB1, TNF-α, and RAGE (all 1:1000 in 5% BSA, Biosesang, Seongnam, Korea) overnight at 4°C.

Techniques: Immunohistochemistry, Staining, Immunohistochemical staining, Amplification, Saline, Comparison

Journal: Yonsei Medical Journal

Article Title: Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model

doi: 10.3349/ymj.2016.57.5.1095

Figure Lengend Snippet: The relative mRNA levels in induced atherosclerosis of rabbit iliac artery. Reverse transcription (RT)-PCR analysis of RAGE, HMGB1, and TNF-α mRNA expression in iliac arteries from four groups of rabbits A. Representative data showing the mRNA expressions of RAGE, HMGB1, and TNF-α in iliac arteries from four groups of rabbits (normalized with GAPDH as the house keeping gene) B, C, and D. The data in the bar graph are quantified ratios of the signal for RAGE, HMGB1, and TNF-α to that for GAPDH set at fold increase. Data are presented as the mean±SEM. * p <0.05, compared with the Saline group, † p <0.05, compared with the Oil group. RAGE, receptor for advanced glycation end products; HMGB1, high-mobility group protein B1; TNF-α, tumor necrosis factor-α; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SEM, standard error of the mean.

Article Snippet: Membranes were blocked by a 5% skim milk (Noble Bio, Hwaseong, Korea) dilution in Tris-Buffered Saline and Tween 20 (TBS-T) at room temperature for 1 hour, and were subsequently washed three times in TBS-T. Membranes were incubated in TBS-T with primary antibodies against HMGB1, TNF-α, and RAGE (all 1:1000 in 5% BSA, Biosesang, Seongnam, Korea) overnight at 4°C.

Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Expressing, Saline

Journal: Yonsei Medical Journal

Article Title: Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model

doi: 10.3349/ymj.2016.57.5.1095

Figure Lengend Snippet: The relative protein levels in induced atherosclerosis of rabbit iliac artery. Western blot analysis of RAGE, HMGB1 and TNF-α mRNA expressions in iliac artery from four groups of rabbits A. Representative data showing the protein expressions of RAGE, HMGB1 and TNF-α levels in iliac arteries from four groups of rabbits (normalized with GAPDH as the house keeping gene) B, C, and D. The data in the bar graph are quantified ratios of the signal for RAGE, HMGB1, and TNF-α to that for GAPDH set at fold increase. Data were presented as the mean±SEM. * p <0.05, compared with the Saline group, † p <0.05, compared with the Oil group, ‡ p <0.05, compared with the TNF-α group. RAGE, receptor for advanced glycation end products; HMGB1, high-mobility group protein B1; TNF-α, tumor necrosis factor-α; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SEM, standard error of the mean.

Article Snippet: Membranes were blocked by a 5% skim milk (Noble Bio, Hwaseong, Korea) dilution in Tris-Buffered Saline and Tween 20 (TBS-T) at room temperature for 1 hour, and were subsequently washed three times in TBS-T. Membranes were incubated in TBS-T with primary antibodies against HMGB1, TNF-α, and RAGE (all 1:1000 in 5% BSA, Biosesang, Seongnam, Korea) overnight at 4°C.

Techniques: Western Blot, Saline